BM and the effect on DNA cleavage induced by H2O2 UV-photolysis was investigated. cytotoxicity and DNA damage in human non-immortalized fibroblasts. from CP, PK, WS and the effect on DNA cleavage induced by H2O2 UV- photholysis. cytotoxicity and DNA damage in human non-immortalized fibroblasts. methanol extract of BM and the effect on DNA cleavage induced by H2O2 UV- photolysis cytotoxicity and DNA damage in human non-immortalized fibroblasts.

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Solid tumors modulate their environment to keep non-malignant stromal cells in a tumor-promoting state. To discover agents capable of blocking CAF differentiation, we developed a high content immunofluorescence-based assay to screen repurposed chemical libraries utilizing fibronectin expression as an initial CAF marker. Overall, these results strongly cytotoxicigy the hypothesis that human colon cancer cells demonstrate greater steady-state levels of intracellular hydroperoxides, relative to normal colon epithelial cells and fibroblasts.

Triptonide treatment strongly inhibited the colony formation- migration- and invasion-promoting capacities of GCAFs. Ethanol exposure induces the cancer-associated fibroblast phenotype and lethal tumor metabolism.

Free radical scavenging capacity and protective effect of Bacopa monniera L. on DNA damage.

The pesticide atrazine does not bind to or activate the classical estrogen receptor ERbut it up-regulates the aromatase activity in estrogen-sensitive tumor cells.

In this study, we searched for differences in the secretome of CAFs and normal oral fibroblasts NOF using mass spectrometry-based proteomics and biological network analysis. In this regard, the functional crosstalk between cancer cells and the tumor microenvironment has received considerable attention in recent years. Cancer-associated fibroblasts CAFs play important roles in cancer progression through their complex interactions with cancer cells.

Clinical relevance and prognostic value of telomere length were investigated on tissue microarrays of surgically treated HCC patients. All biochemical determinations were normalized to the protein content using the method of Lowry et al [ 22 ].

We cultured and immortalized CAFs and NPFs, then compared their effect on epithelial malignant transformation by using in vitro co-culture, soft agar assay, and a mouse renal capsule xenograft model. Cancer cells, relative to normal cells, demonstrate increased sensitivity to glucose deprivation-induced cytotoxicity. An alternative role of estrogen in mammary carcinoma development. The presence of podoplanin-positive CAFs was associated with smoking history, solid predominant subtype, and lymph node metastasis.


With a newly developed device, CAFs was proven to promote cell adhesion to human brain microvascular endothelial cells, in vitro BBB permeability and transmigration and colony formation of breast cancer cells. To investigate the difference of miRNA expression levels of cancer-associated fibroblasts CAFs and normal fibroblasts NFs in human breast cancer microenvironment and its effect on the biological features of CAFs.

Histological inspection of visually normal tissue adjacent to neoplastic lesions often reveals multiple foci of cellular abnormalities.

Furthermore, the combination of cisplatin and pirfenidone in NSCLC cells A and H cells leads to increased apoptosis and synergistic cell death. These myofibroblasts promote invasion and metastasis of cancer cells.

We aimed to evaluate the potential of atrazine to trigger GPER-mediated signaling in cancer cells and cancer-associated fibroblasts CAFs. We found this inhibition to hold true for multiple fibroblast cell lines. Zoledronic acid impairs stromal reactivity by inhibiting M2-macrophages polarization and prostate cancer-associated fibroblasts.

We investigated the characteristics and functions of CAFs in diffuse-type gastric cancers DGCs by analyzing features of their genome and gene expression patterns. Heterogeneity in 2-deoxy-D-glucose-induced modifications in energetics and radiation responses of human tumor cell lines. The cancer stroma, including cancer-associated fibroblasts CAFsis known to contribute to cancer cell progression and metastasis, suggesting that functional proteins expressed specifically in CAFs might be candidate molecular targets for cancer treatment.

These results show that cancer cells relative to normal cells demonstrate increased steady-state levels of ROS reactive oxygen species; i.

Specific inhibition of fibroblast activation protein FAP -alpha prevents tumor progression in vitro. The objective of our study was to investigate whether cancer-associated fibroblasts CAFs play crucial roles in breast cancer brain metastasis.

In this study, we investigated the role of mechanical stimuli on human prostatic fibroblasts using a microfluidic platform vibroblasts was adapted for our experiments and further developed for both repeatable performance among multiple assays and for compatibility with high-resolution confocal microscopy.

Estrogen is likely to affect tumor associated stroma and contributes to mammary carcinoma development through CAFs.

In addition 2DG sensitizes cancer cells to cisplatin and adriamycin [ 343638 ], which are thought to induce oxidative stress.

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Hypermethylation in the promoter region of miR genes was accounted for its downregulation. This study aimed to delineate the variation and prognostic value of nonimmodtalized length in HCC.

In this study, we found that polysaccharides extracted from Dictyophora indusiata may affect the micro-environment of tumours and inhibit the growth of the tumours. On the basis of our data, atrazine should be included among the environmental contaminants that may elicit estrogenic activity through GPER-mediated signaling.

cancer-associated fibroblasts cafs: Topics by

These results strongly suggest that depletion of NADPH levels in colon and breast carcinoma cells fibroblatss contribute to glucose deprivation-induced cytotoxicity and oxidative stress in cancer cells. The interrelationship between malignant epithelium and the underlying stroma is of fundamental importance in tumour development and progression.

Triptonide inhibits the pathological functions of gastric cancer-associated fibroblasts. The number of cells excluding the dye viable and cell stained by the dye non-viable were counted.

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AR expressed in mesenchyme is necessary and sufficient for prostate development while loss of stromal AR is predictive of prostate cancer progression. It has been largely reported that the classical estrogen receptor ERas well as the G protein estrogen receptor GPER, previously known as GPR30 can exert a main role in the development of breast tumors. Inhibition of tumour-associated fibroblastswhich provide nourishment and support to tumour cells, is a novel and promising anti-tumour strategy.

Moreover, we show that E2 and G-1 down-regulate through miR the onco-suppressor Runx1 and increase cell cycle progression. Moreover, we show that both pro-inflammatory signaling by CAFs and leukocyte infiltration of tumors are enhanced in invasive ductal carcinoma as compared with ductal carcinoma in situ.

These results are confirmed in a metastatic xenograft PCa mouse model in which ZA-induced stromal normalization impairs cancer-stromal cells crosstalk, resulting in a significant reduction of primary tumour growth and metastases. Next, we found that the A20 down-regulation, as well as the up-regulation of c-Rel induced by miR were no longer evident using LNA-i-miR